In situ transcription in cells and tissues
US5021335A · kind A · utility
89Cited by
1References
2Claims
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Key dates
| Filing date | Jun 17, 1988 |
| Grant date | Jun 4, 1991 |
| Priority date | — |
| Expiry date | Jun 17, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/48
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with reverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.