Non-intrusive process for monitoring the production of recombinant proteins
US5021337A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 15, 1988 |
| Grant date | Jun 4, 1991 |
| Priority date | — |
| Expiry date | Jul 15, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/68
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process is described for monitoring the production of recombinant proteins. It is based on the discovery that infrared spectra of E. coli strains and transformants which overproduce recombinant proteins can be measured as a function of pressure, and a pressure-induced distinct shifting pattern can be observed in specific spectral parameters of transformants. In particular, the difference in the pressure-induced shift of the amide III band, between the E. coli cells and the transformants over-producing the recombinant proteins provides an efficient and non-intrusive technique for on-line monitoring of the production of recombinant proteins in E. coli.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.