Immobilzation of lipase for resolving racemates of esters of racemic alcohols

US5021345A · kind A · utility

Assignee

• BASF SE

Inventors

• Dieter Urban · Speyer, DE
• Wolfgang Ladner
• Axel Paul · Lampertheim, DE

Key dates

• Filing date: Nov 4, 1988
• Grant date: Jun 4, 1991
• Expiry date: Nov 4, 2008

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N11/082
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Lipase is immobilized by mixing a substrate, a crude porcine pancreatic lipase, an aqueous buffer solution at pH 5-9 and one or more water-soluble polyhydric aliphatic alcohols having from 2 to 6 carbon atoms and from 2 to 6 hydroxyl groups at a temperature of from 0.degree. to 40.degree. C. to form a mixture, allowing the mixture to stand for from 1 minute to 3 days, filtering the mixture to produce a filter cake and washing the cake to obtain the immobilized lipase. The substrate is a pulverulent, insoluble, only slightly swellable copolymer of one or more N-vinyllactams of 4 to 6 carbon atoms with a cyclic amide which contains two or more ethylenically unsaturated, copolymerizable groups, one or more of which are bonded directly to amide nitrogen. The polyhydric aliphatic alcohol can be combined with one or more water-soluble monohydric alcohols having from 1 to 4 carbon atoms. The filter cake can be treated with a crosslinking agent such as a water-soluble glycidyl ether which is prepared by reacting an epoxide with a polyfunctional alcohol to form an adduct and reacting the adduct with epichlorohydrin followed by cyclization to give an epoxide. The immobilized lipase is used f…