Method for gene splicing by overlap extension using the polymerase chain reaction
US5023171A · kind A · utility
152Cited by
2References
3Claims
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Key dates
| Filing date | Aug 10, 1989 |
| Grant date | Jun 11, 1991 |
| Priority date | — |
| Expiry date | Aug 10, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6881
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for joining two DNA molecules by first amplifying them by means of polymerase chain reactions (PCR) carried out on each molecule using oligonucleotide primers designed so that the ends of the resultant PCR products contain complementary sequences. When the two PCR products are mixed, denatured and reannealed, the single-stranded DNA strands having the complementary sequences at their 3' ends anneal and then act as primers for each other. Extension of the annealed area by DNA polymerase produces a double-stranded DNA molecule in which the original molecules are spliced together.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.