Method of somatotropin naturation
US5023323A · kind A · utility
12Cited by
12References
15Claims
0Family size
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Inventor
Key dates
| Filing date | Sep 12, 1990 |
| Grant date | Jun 11, 1991 |
| Priority date | — |
| Expiry date | Sep 12, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the solubilization and naturation of somatotropin from refractile bodies produced by r-DNA technology wherein the refractile bodies are dissolved in an aqueous solution comprising a chaotropic agent such as urea or guanidine hydrochloride and a soluble surfactant such as sodium dodecylsulfate. The solubilized protein is exposed to mild oxidation for a time sufficient to allow the protein to form disulfide bonds and refold to its native conformation. The presence of the surfactant suppresses the formation of somatotropin dimers and aggregates and results in higher yields of the desirable monomeric form of the protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.