Regulatory region cloning and analysis plasmid for bacillus
US5024943A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 4, 1986 |
| Grant date | Jun 18, 1991 |
| Priority date | — |
| Expiry date | Nov 4, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel constructs in plasmids are provided for evaluating the efficiency of expression and secretion of structural genes. The constructs provide for transcriptional and translational regulatory regions, a signal sequence and structural gene, which also may be readily excised and substituted, so as to allow for mixing and matching of regulatory regions, signal sequences and genes to evaluate regions for use in the expression of a desired peptide. Particularly, synthetic regions are provided which may be used with other synthetic regions or wild-type regions. Plasmids are provided for screening Bacillus genomic sequences for regulatory regions, particularly promoters employing a structural gene secreting an enzyme which can produce a product which allows for visual detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.