Biospecific multianalyte assay method
US5028545A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 9, 1988 |
| Grant date | Jul 2, 1991 |
| Priority date | — |
| Expiry date | Jun 9, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/14
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In a biospecific multianalyte assay the use of microspheres and fluorescent labels with substantially different fluorescence decay times, is combined. The assay is performed in a suspension of microspheres in the form of a pool of different microsphere categories, where the categories represent different analytes. The microspheres belonging to the respective categories are first coated with a specific reactant, i.e. the microspheres function as a solid support for the reactant and for a biospecific reaction. Fluorescent labels having a short decay time are used to identify the category of each individual microsphere, while fluorescent labels having a long decay time are used to determine the concentration of a particular analyte on the microsphere by means of the biospecific reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.