Means and method for enhancing nucleic acid hybridization
US5030557A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 24, 1987 |
| Grant date | Jul 9, 1991 |
| Priority date | — |
| Expiry date | Nov 24, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The binding of a nucleic acid probe with its complementary sequence in a targeted, single stranded nucleic acid is affected by the secondary and tertiary structure of the target nucleic acid. The rate and extent of hybridization of the probe with the targeted nucleic acid can be increased by the use of "helper" oligonucleotides. Helper oligonucleotides are selected to bind to the target nucleic acid and impose a different secondary and tertiary structure on the target to facilitate the binding of the probe to the target. The resulting hybrid of probe and target nucleic acid also exhibits a higher T.sub.m than the hybrid which results from addition of the probe alone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.