Bacterial hypersecretion using mutant repressor sequence
US5030563A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 26, 1990 |
| Grant date | Jul 9, 1991 |
| Priority date | — |
| Expiry date | Jan 26, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/10322
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved bacterial host cell useful for the inducible production and secretion in high yields of a heterologous protein is provided which contains a gene encoding the heterologous protein operatively linked to a secretory leader-encoding sequence and to an expression control sequence which contains a promoter region; and a second DNA sequence encoding a repressor capable of binding to said promoter region. The cell contains at least a mutation in the repressor binding region of the promoter or a mutation in the promoter binding region of the repressor-encoding sequence; or mutations in both regions. These mutation(s) lower the frequency of transcriptional induction by the promoter from the observed with the wild-type promoter and/or repressor-encoding sequence, resulting in higher yields of secreted heterologous protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.