Microorganism immobilized in a gel prepared from polyvinyl alcohol and a polysaccharide
US5034324A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 26, 1988 |
| Grant date | Jul 23, 1991 |
| Priority date | — |
| Expiry date | Jul 26, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A hydrated gel of high water content and high porosity containing an immobilized microorganism is prepared by dropwise addition of an aqueous solution containing a microorganism, polyvinyl alcohol and a polysaccharide to an aqueous solution containing polyvalent metal ions to gel the polysaccharide and form spherical gel beads, and subjecting the beads to at least one cycle of freezing and thawing to gel the polyvinyl alcohol. The polysaccharide can be sodium alginate and the polyvalent metal ions can be provided by calcium chloride. Freezing is at a temperature not higher than -5.degree. C., and the cycle of freezing and thawing is preferably repeated at least two times.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.