Novel cross-linking agents

US5034514A · kind A · utility

Assignee

• Cetus Corporation · US

Inventors

• Danute E. Nitecki · Berkeley, US
• Margaret Moreland · Berkeley, US

Key dates

• Filing date: Oct 12, 1988
• Grant date: Jul 23, 1991
• Expiry date: Oct 12, 2008

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07D213/71
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Bifunctional chemicals useful for linking aldehyde containing moieties to moieties containing functionalities which are or can be reactive with sulfhydryl are claimed and have the formula: EQU L--S--(CH.sub.2).sub.n1 --CONH--SPACER--NH--X wherein PA1 L is a leaving group selected from --H or --S--Ar, wherein Ar represents optionally substituted phenyl or pyridyl; PA1 n.sub.1 =2-4; PA1 X is selected from: --CO--Y--CONHNH.sub.2, a hydrazide; ##STR1## a hydrazine; ##STR2## a hydrazine; --CO--Y--NH--CONHNH.sub.2, a semicarbazide; and --CO--Z--NH--CSNHNH.sub.2, a thiosemicarbazide; PA1 wherein Y is alkylene or oxaalkylene and Z is alkylene or a polypeptide residue briding the N-terminal amino group and C-terminal carboxy group thereof; PA1 the SPACER is oxaalkylene or oxaalkylene substituted with hydroxyl and specifically includes residues having formulas selected from PA2 a) --(CH.sub.2).sub.n2 --O--(CH.sub.2).sub.n2 --O--(CH.sub.2).sub.n2 -- wherein each n2 is independently 2-4 and PA2 b) ##STR3## wherein n3 is 2-6. Also claimed are hydrazides, semicarbazides, and thiosemicarbazides of .omega.-mercaptocarboxylic acids which lack SPACER diamine.