Process and test carrier for the determination of an analyte

US5037736A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Helmut Freitag · Indianapolis, US
• Hans-Erich Wilk · Mannheim, DE
• Anselm Rothe · Birkenau, DE

Key dates

• Filing date: Nov 18, 1987
• Grant date: Aug 6, 1991
• Expiry date: Nov 18, 2007

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/823
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a process for the determination of an analyte in a body fluid, in which there are used two binding components capable of specifically binding with one another, one of the binding components being enzyme-labelled and not carrier-fixed and the other binding component being carrier-fixed. The process contains a step in which the binding components are incubated with one another so that binding reaction takes place. The amount of enzyme-labelled binding component not bound to the carrier-fixed binding component is a measure of the concentration of the analyte which is determined by allowing the labelling enzyme to act upon a substrate producing a detection signal. During the specific binding reaction, incubation is carried out simultaneously with a non-fixed substrate of the labelling enzyume which does not produce a detection signal and with a carrier-fixed substrate of the labelling enzyme which produces a detection signal, the substrate not producing a detection signal being so chosen with regard to the amount used and affinity to the labelling enzyme in relation to the amount of the substrate producing a detection signal and its affinity to the labell…