Monoclonal antibody and method for diagnosing grape disease employing same
US5037755A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 2, 1987 |
| Grant date | Aug 6, 1991 |
| Priority date | — |
| Expiry date | Dec 2, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/1264
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A monoclonal antibody specific to Agrobacterium tumefaciens biovar 3. A method of diagnosing Agrobacterium tumefaciens biovar 3 associated grapevine disease comprising: (1) culturing bacteria from grapevine tissue suspected of being infected with Agrobacterium tumefaciens biovar 3; (2) reacting the bacteria with a monoclonal antibody specific to Agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaciens biovar 3 and the monoclonal antibody; and (3) detecting the presence of the antigen-antibody complex. A method for diagnosing Agrobacterium tumefaciens biovar 3 associated grapevine disease from crown gall tissue comprising: (1) preparing separate suspensions of ground gall tissue to be diagnosed and of ground wood of the same cultivar as a control; (2) separately reacting specific to Agrobacterium tumefaciens biovar 3 under conditions sufficient to form an antigen-antibody complex between antigens specific to Agrobacterium tumefaciens biovar 3 and the monoclonal antibody; (3) assaying for the presence of the antigen-antibody complex ; and (4) comparing the assay results for the gall tissu…
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