Process for determination of an analyte and reagent therefor

US5037764A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Hans-Erich Wilk · Mannheim, DE
• Helmut Freitag · Indianapolis, US
• Josef Burg · Weilheim, DE
• Johann Berger · Alfdorf, DE

Key dates

• Filing date: Jul 16, 1987
• Grant date: Aug 6, 1991
• Expiry date: Jul 16, 2007

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54306
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a process for the detection of an analyte, wherein a sample containing the analyte is incubated either with a labelled binding partner and the immobilized analyte or an immobilized analyte analogue or with the labelled analyte or analyte analogue and an immobilized binding partner, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte, and the labelled binding partner or the labelled analyte or the labelled analyte analogue is present in insufficiency compared with the concentration of the analyte to be detected. The present invention also provides an agent for carrying out this process, wherein it contains either the immobilized analyte or an immobilized analyte analogue and a labelled binding partner or the labelled analyte or a labelled analyte analogue and an immobilized binding partner, as well as optionally an appropriate buffer system and further conventionally used adjuvants, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte.