Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5038852A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 1990 |
| Grant date | Aug 13, 1991 |
| Priority date | — |
| Expiry date | Mar 14, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/115831
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.