Procaryotic xylose isomerase muteins
US5041378A · kind A · utility
23Cited by
4References
1Claims
0Family size
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Key dates
| Filing date | Aug 11, 1987 |
| Grant date | Aug 20, 1991 |
| Priority date | — |
| Expiry date | Aug 11, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/886
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Xylose isomerase (XI) muteins useful in the conversion of glucose to fructose or xylose to xylulose are obtained in usable amounts by protein structural and recombinant DNA methods, including x-ray crystallography, cloning, computer graphic modeling and site-directed mutagenesis and expression of the bacterial DNA sequences encoding native procaryotic xylose isomerase. These native sequences are altered to encode the xylose isomerase muteins having improved catalytic function and/or thermostability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.