Method of preparing a high-purity, virus safe, biologically active transferrin preparation

US5041537A · kind A · utility

Assignee

• Biotest Pharma GmbH · DE

Inventors

• Ulf Bethke · Babenhausen, DE
• Norbert Kothe · Kronberg, DE
• Dieter Rudnick · Dreieich, DE
• Wolfgang Moller · Lübeck, DE
• Michael Kloft · Weiterstadt, DE

Key dates

• Filing date: Sep 27, 1988
• Grant date: Aug 20, 1991
• Expiry date: Sep 27, 2008

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

To provide a simple method of preparing biologically active transferrin that can be employed on an industrial scale and that will result in extremely pure and natural transferrin containing no viruses and appropriate for both in-vitro and in-vivo applications, the .gamma.-globulins are precipitated from the fraction containing the transferrin, the precipitant is removed from the residual liquid by ultrafiltration or gel filtration, the liquid is adjusted to a prescribed ionic concentration and protein concentration and (a) treated with .beta.-propiolactone and the solution is subjected to ultraviolet radiation or (b) treated with specific detergents and subjected to ion-exchange chromatography, and the transferrin is concentrated and filtered sterile.