Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5043272A · kind A · utility
311Cited by
1References
50Claims
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Assignee
Inventor
Key dates
| Filing date | Apr 27, 1989 |
| Grant date | Aug 27, 1991 |
| Priority date | — |
| Expiry date | Apr 27, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
According to this invention, a process for substantially amplifying template nucleic acid present in a sample is described, wherein said amplification may be performed without prior knowledge of specific sequences, which process comprises apposition of random oligonucleotide primers to said template nucleic acid under conditions such that extension products of said primers are synthesized which are complementary to said template nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.