Method for purifying low molecular weight compounds of peptide or pseudo-peptide structure
US5043423A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 1990 |
| Grant date | Aug 27, 1991 |
| Priority date | — |
| Expiry date | Aug 28, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/02
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
This invention firstly provides a method for purifying particular compounds of peptide or pseudo-peptide structure in which the number of protonable basic functions is greater than the number of acid functions and which have a molecular weight of less than 1000 daltons, by ion exchange displacement chromatography. In the method of the present invention the stationary phase used is a cationic exchange resin or a cross-linked polymer matrix activated with acid groups; the transporter solvent used is water if the compound to be purified already possesses at least one net positive charge, or aqueous dilute solutions of inorganic or strong organic acids which protonate the basic groups of the peptide or pseudo-peptide to be separated without modifying the structure of the peptide compound, such as acetic acid, trifluoroacetic acid, formic acid, hydrochloric acid or sulphuric acid; the displacer compound used is a triethylenetetraammonium salt.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.