Method and apparatus for the production of TGF-.beta.
US5043431A · kind A · utility
4Cited by
6References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 11, 1989 |
| Grant date | Aug 27, 1991 |
| Priority date | — |
| Expiry date | Sep 11, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/495
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Transforming growth factor .beta. (TGF-.beta.) is produced in relatively large quantities and at a relatively high purity by fermentation in a perfusion microcarrier reactor. Conditioned media from the reactor is first treated to provide the active form of TGF-.beta. and subsequently purified by cation exchange chromatography followed by hydrophobic interaction chromatography. Optionally, nucleic acids complexed with the TGF-.beta. may be removed while the protein is bound to the cation exchange resin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.