Analytical methods utilizing reducible components
US5045477A · kind A · utility
5Cited by
10References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 28, 1989 |
| Grant date | Sep 3, 1991 |
| Priority date | — |
| Expiry date | Apr 28, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2304/26
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Certain reducible compounds are useful in analytical compositions, elements and methods, e.g. for assays of bacterial cells. These compounds comprise a moiety which provides a detectable species (e.g. a dye) when released from the compound in an environment of pH 9 or less (i.e. physiological pH). Structurally, the reducible compounds are aromatic derivatives or quinones having suitable substituents which promote varying amounts of moiety release at physiological pH. When reduced at about pH 7, the preferred compounds release at least 50% of the available detectable species within 30 minutes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.