Thrombin-binding substance and process for its preparation
US5047503A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 10, 1987 |
| Grant date | Sep 10, 1991 |
| Priority date | — |
| Expiry date | Jul 10, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/834
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Thrombin-binding substances are obtained by fractionating human urine by ion-exchange chromatography, affinity chromatography using a thrombin-bound carrier, immune adsorption column chromatography, gel filtration, and/or molecular-weight fractionation. One of the substances has a molecular weight of 46,500.+-.6,000 in reduced condition and 39,000.+-.10,000 in unreduced condition by SDS PAGE and an isoelectric point at pH 5.0-5.3, while the other has a molecular weight of 40,000.+-.8,000 in reduced condition and 31,000.+-.10,000 in unreduced condition by SDS PAGE and an isoelectric point at pH 4.9-5.7. They have strong affinity to thrombin. They are capable of promoting the thrombin catalyzed activation of protein C and prolong clotting time. They are stable to denaturing agents (urea and sodium dodecylsulfate).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.