Patent · US Expired

Method for the purification of factor XIII by affinity chromatography

US5047506A · kind A · utility

2Cited by

2References

14Claims

0Family size

Assignee

Behringwerke Aktiengesellschaft · DE

Inventors

Hartmut Lobermann · Schallstadt, DE
Jürgen Römisch · Marburg, DE
Werner Stuber · Lahntal, DE

Key dates

Filing date	Mar 9, 1989
Grant date	Sep 10, 1991
Priority date	—
Expiry date	Mar 9, 2009

Classification

Technology area (CPC A)Human Necessities
CPC primaryA61K38/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The invention relates to a method for the purification of the a subunit of factor XIII by affinity chromatography, to a therapeutic composition containing the latter, and to the use of the therapeutic composition. Factor XIII has hitherto been purified either by methods which are technically very elaborate or else by use of toxic affinity chromatography materials. The invention has the aim of providing an improved method for the purification of the a subunit of factor XIII. Factor XIII is obtained according to the invention by a method in which the a subunit of factor XIII is reversibly bound to a matrix suitable for disulfide exchange reactions and is removed from the matrix by reaction with a reducing agent. The method according to the invention makes it possible to provide the biologically active a subunit of factor XIII in high purity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.