Plasmids for the increased production of penicillin G amidase

US5053335A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Gunter Schumacher · Altenkirchen, DE
• Peter Buckel · Bernried, DE

Key dates

• Filing date: Jan 27, 1989
• Grant date: Oct 1, 1991
• Expiry date: Jan 27, 2009

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/84
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a plasmid suitable for the expression of penicillin G amidase, wherein it carries an incomplete penicillin G amidase gene in which the first 78 bases of the translated region on the 5' end of the complete gene are missing. This incomplete penicillin G amidase gene can also be present incorporated in the form of two separate fragments which commence with bases 79 and 868, respectively, counting from the translated region on the 5' end of the gene and displaying a deletion of the coding bases 705 to 867. The present invention also provides a process for the production of these plasmids and is also concerned with the use thereof for obtaining penicillin G amidase.