Method for the determination of the activity of serine proteases or serine protease inhibitors
US5057414A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 1, 1988 |
| Grant date | Oct 15, 1991 |
| Priority date | — |
| Expiry date | Jul 1, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/8132
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for the determination of the activity of serine proteases and serine protease inhibitors in plasma or other biological fluids. The only methods hitherto available for rapid and accurate determination of the content of serine proteases or serine protease inhibitors have been very time-consuming and methodologically demanding. In the method according to the invention for the determination of the activity of serine proteases or serine protease inhibitors, specific inhibitors are inactivated by addition of at least one oxidizing agent and, where appropriate, at least one detergent. The addition of an omega-aminocarboxylic acid enhances and observed effects when the serine protease is plasmin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.