Thin film membrane enzyme/coemzyme reactor and method of using same
US5057421A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1989 |
| Grant date | Oct 15, 1991 |
| Priority date | — |
| Expiry date | Apr 7, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An enzyme reactor system is provided based on the entrapment of a coenzyme-requiring enzyme, a coenzyme, and a regeneration enzyme in a hydrogel layer coated on a support, and confined by an ultraporous thin film semipermeable membrane. The diffusion barrier confines the coenzyme-requiring enzyme, coenzyme, and regeneration enzyme but lets substrate and reaction products, exclusive of coenzyme, diffuse freely into and out of the hydrogel layer. In an alternate embodiment, the support is formed of an ultraporous thin film semipermeable membrane on a microporous or macroporous support, through which the reaction products, exclusive of coenzyme, can diffuse freely, but through which neither coenzyme-requiring enzyme, coenzyme, regeneration enzyme, nor substrate can pass. In this embodiment, the product is recovered in high purity, free of substrate, coenzyme-requiring enzyme, coenzyme, and regeneration enzyme. A constant supply of coenzyme for the enzyme reaction is assured by initially charging the hydrogel layer with an adequate supply of coenzyme. The problem of coenzyme depletion is solved by including a second enzyme in the hydrogel layer which regenerates the used coenzyme, thus…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.