Stabilized lyophilized mammalian cells and method of making same
US5059518A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 20, 1988 |
| Grant date | Oct 22, 1991 |
| Priority date | — |
| Expiry date | Oct 20, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/101666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of lyophilizing mammalian cells to produce preserved human cells, hybridoma cell lines, tissue cells, and control cells for immunoassays and other hematological measurements. Prior to freezing and lyophilizing, the prepared mammalian cell pellet is suspended in a solution of trehalose in an isotonic fluid prepared at a specified optimal concentration and incubated at room temperature for a designated time period. The lyophilized cells when rehydrated, retain their optimal physiological characteristics suitable for use as an analytical control and retain said characteristics after storage at 2.degree.-8.degree. C. for a period in excess of five (5) months.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.