Patent · US Expired

Rhizobium japonicum 191 NODD-related genes

US5059534A · kind A · utility

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Inventor

Key dates

Filing dateJul 17, 1989
Grant dateOct 22, 1991
Priority date
Expiry dateJul 17, 2009

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/878
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The isolation and characterization of nodD-related genes in soybean nodulating Rhizobium japonicum is described. In R. japonicum USDA 191 two such genes have been identified, which although related in structure, have different functional properties. These nodD genes are functionally distinct from each other and from those nodD genes of other strains of Rhizobium that have been isolated and characterized to date. In particular, nodD-r1 has been found to affect nodulation on soybean and to be associated with exopolysaccharide production. In contrast, nodD-r2 affects nodulation on the tropical legume siratro. The coding sequences of both nodD genes are provided. The promoter regions of the nodD genes have also been isolated. The genes described herein, including both structural genes and their promoter regions, can be used in combination with other genetic constructs to enhance the efficiency and competitiveness of nodulation.

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