Process for the separation and purification of sialyl transferases
US5059535A · kind A · utility
8Cited by
4References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 12, 1989 |
| Grant date | Oct 22, 1991 |
| Priority date | — |
| Expiry date | Apr 12, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Specific sialyl transferases (ST) are important enzymes in the manufacture of carbohydrate moieties useful for pharmacological purposes and for diagnostic and clinical procedures. The invention offers simplified methods to isolate highly specific forms of these ST enzymes by taking advantage of the affinity of the ST for its acceptor substrate in the presence of the sialyl-CMP analogs, such as CDP. Specifically exemplified are isolation of the alpha 2,3-ST, specific for Le.sup.c or LacNAc, and alpha 2,6-ST specific for LacNAc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.