Preparation of gel-filled separation columns
US5061355A · kind A · utility
43Cited by
4References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 14, 1990 |
| Grant date | Oct 29, 1991 |
| Priority date | — |
| Expiry date | Jun 14, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of preparing a microcapillary gel separation column for electrophoresis comprises: pretreatment of a tube with a bifunctional reagent to provide polymer bonding sites on the inner wall of the tube; filling the tube with a monomer solution a crosslinking agent, and a photoinitiator; forming a polymer plug by illuminating an initiation section of the tube; growing the plug by moving the tube gradually past the illumination source; and stopping tube motion when the desired length of gel is attained. Provision is made for monitoring polymerization and adjusting tube movement speed to avoid gaps in the growing polymer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.