Process for preparing a carrier useful in immunoassays by deposition of a complex of a specifically binding substance with hydrophobic protein, and the resulting carrier

US5061640A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Wilhelm Tischer · Peißenberg, DE
• Josef Maier · Eichstätt, DE
• Rolf Deeg · Seeshaupt, DE

Key dates

• Filing date: Nov 24, 1987
• Grant date: Oct 29, 1991
• Expiry date: Nov 24, 2007

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/78
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a process for the preparation of a specifically bindable protein substance bound to an insoluble carrier material, especially for use in a heterogeneous analysis process, wherein a soluable protein with a molecular weight above about 500,000, which is more hydrophobic than the specifically bindable substance, is coupled to the specifically bindable substance and then the conjugate of reaction component and protein is adsorbed on a hydrophobic solid phase. The present invention also provides a carrier material for use in solid phase immunoassays including a hydrophobic solid phase which is adsorbed on a conjugate of a hydrophobic protein with a molecular weight above about 500,000 and of a specifically bindable protein or protein containing substance.