Method of isolating blood-clotting factor IX

US5061789A · kind A · utility

Assignee

• Biotest Pharma GmbH · DE

Inventors

• Wolfgang Moller · Lübeck, DE
• Michael Kraus · Ehrenkirchen, DE

Key dates

• Filing date: May 1, 1990
• Grant date: Oct 29, 1991
• Expiry date: May 1, 2010

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y304/21022
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of isolating blood-clotting Factor IX. A solution containing the Factor IX is adsorbed onto an adsorbent carrying .alpha.-hydroxylamine groups, and the factor is eluted. The Factor IX from the eluate is adsorbed onto a matrix carrying sulfated carbohydrates, with the conductivity adjusted to 13-17 mS/cm, and eluted with a salt gradient. The pure Factor IX is concentrated and freeze-dried.