Performing an enzyme-catalyzed reaction
US5063149A · kind A · utility
1Cited by
2References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 4, 1989 |
| Grant date | Nov 5, 1991 |
| Priority date | — |
| Expiry date | Aug 4, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P1/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A process for performing an enzyme-catalyzed reaction in a reaction mixture comprising two phases, a first aqueous phase containing an enzyme which is not accessible and a second substantially non-aqueous phase containing a non-water miscible solvent wherein a compound derived from fluorescein or a substituted fluorescein is present for the purpose of measuring aqueous pH by monitoring spectroscopic properties. Novel compounds derived from fluorescein or a substituted fluorescein which may be used in the process and a method for producing some of the novel compounds are also claimed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.