Method and device for producing a controllable and reproducible temperature gradient and use thereof
US5066377A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 29, 1990 |
| Grant date | Nov 19, 1991 |
| Priority date | — |
| Expiry date | Jun 29, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/303
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and a device for producing a controllable and reproducible temperature gradient in a predetermined direction of heat-conducting plates for the separation of mixtures of substances in sheet-shaped separating media wherein one edge of a plate is heated by means of one or more controllable heating means and the opposite edge of the plate is cooled by one or more controllable cooling means. In this way, the energy flows through the heating means and of the cooling means are greater than the energy flow across the plate. The method and device are suitable for separating mixtures of substances wherein at least one component undergoes a thermal conversion within the temperature range of the temperature gradient. Therefore, they are suitable for the detection of and differentiation between viroids, satellite RNAs, viruses containing double-strand or circular nucleic as well as for the analysis of protein-nucleic acid complexes and for the analysis of mutations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.