Thermal control for capillary electrophoresis apparatus
US5066382A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 25, 1990 |
| Grant date | Nov 19, 1991 |
| Priority date | — |
| Expiry date | Jan 25, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44708
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electrophoresis instrument includes a capillary tube mounted in an air cooled cartridge. The cartridge also supports a spherical lens which is part of the optical detection apparatus. The cartridge rests in a manifold which includes the sample and buffer reservoirs. The temperature of the capillary tube is controlled by measuring the electrical resistance of the capillary tube during the electrophoresis process and then cooling or heating the cartridge by circulating temperature controlled air over the tube. The optical path associated with the instrument is a fiber optic bundle bifurcated close to dual detectors into a reference arm and a sample arm so as to provide similar reference and sample optical paths. The instrument may be used for temperature control for gradient electrophoresis and also, a neutral marker for determining electro-osmotic flow may be detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.