Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins
US5066426A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 23, 1984 |
| Grant date | Nov 19, 1991 |
| Priority date | — |
| Expiry date | Aug 23, 2004 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescence polarization techniques to provide a means for determining the amount of a specific ligand present in a sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.