Methods for generating single stranded DNA by the polymerase chain reaction
US5066584A · kind A · utility
184Cited by
5References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 23, 1988 |
| Grant date | Nov 19, 1991 |
| Priority date | — |
| Expiry date | Sep 23, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Single stranded DNA can be generated by the polymerase chain reaction using two oligonucleotide primers, one persent in a limiting concentration. The single stranded DNA is useful in procedures involving utilizing nucleic acid probes and for purposes of nucleic acid sequencing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.