Gas driven microprojectile accelerator and method of use
US5066587A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 26, 1990 |
| Grant date | Nov 19, 1991 |
| Priority date | — |
| Expiry date | Jan 26, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8207
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A foreign substance, such as DNA, is injected into plant cells or tissue by a biological ballistical insertion technique. High pressure propellant gas is employed to accelerate the macroprojectile containing microprojectiles coated with the foreign substance. A movable macroprojectile stop mechanism is provided between an upper injection chamber and a lower sample-holding chamber so that multiple shots of macroprojectiles can be made without disrupting the vacuum in the lower chamber. The stop mechanism includes removable stopper plugs for stopping the macroprojectile. A vacuum seal is provided between the stop mechanism and the lower chamber.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.