Method for the purification of interferon
US5066786A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 18, 1988 |
| Grant date | Nov 19, 1991 |
| Priority date | — |
| Expiry date | Jul 18, 2008 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is described for the purification of crude human interferon from solutions containing it, which comprises: PA0 a) the complete adsorption of the crude interferon in a column of siliceous material which has previously been disinfected with an aqueous solution of formaldehyde; PA0 b) the washing of the column with non-pyrogenic, sterile, deionized water; PA0 c) the removal of the extraneous residual proteins by the elution of the column successively with a 1.4 M aqueous solution of NaCl in non-pyrogenic, sterile, deionized water, and with an aqueous solution of acetic acid having a molar concentration of 0.001 M to 0.003 M; PA0 d) the elution of the interferon from the column with an aqueous solution of acetic acid having a molar concentration of from 0.01 to 0.03 M and finally, PA0 e) the recovery and lyophilization of the elution containing the purified interferon. The method enables interferon to be obtained, the purity of which, measured as its specific activity, is at least 1000 times greater than that of the crude interferon, with a yield greater than 85%. The interferon thus purified is particularly useful for treatment in man.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.