Method for the simultaneous determination of DNA sequence variations at a large number of sites, and a kit suitable therefor
US5068176A · kind A · utility
34Cited by
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9Claims
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Key dates
| Filing date | May 1, 1989 |
| Grant date | Nov 26, 1991 |
| Priority date | — |
| Expiry date | May 1, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a method of simultaneously determining DNA sequence variations on a large number of loci, and a kit suitable therefor. The method comprises an electrophoretic separation of restriction fragments in two dimensions on the basis of two independent criterions, namely, length and base pair sequence, and a detection of separated fragments by means of a labelled probe comprising one or more units of minisatellite sequences. Preferred probes are GC-rich minisatellite core sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.