Test carrier for analysis of a sample liquid, method of using a test carrier and process for making a test carrier layer

US5071746A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Hans-Erich Wilk · Mannheim, DE
• Karin Munter · Mannheim, DE
• Rolf Lerch · Ilvesheim, DE

Key dates

• Filing date: Apr 13, 1989
• Grant date: Dec 10, 1991
• Expiry date: Apr 13, 2009

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54391
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a test carrier for the analytical determination of a component of a sample liquid with the help of immunological reagents having a conjugate layer (2, 12), which contains a soluble conjugate of a first immunological binding partner with a labelling, a porous fixing layer (3, 13), which contains a second binding partner in carrier-fixed form binding specifically with the first binding partner, and an optical detection layer (8, 18), wherein the fixing layer (3, 13) is constructed in such a manner that the suction speed with which the liquid is transported in it is so high that the time needed for the spreading out of the liquid in the fixing layer is considerably shorter than the incubation time which is needed for the completion of the specific binding reaction between the first binding partner and the second binding partner and the optical detection layer (8, 18) is so fixed on the test carrier (1, 11) that, in a first position, it is not in contact with the fixing layer (3, 13) making possible a liquid exchange but can be brought into a second position in which a liquid exchange between the fixing layer (3, 13) and the optical detection layer (8, 18…