Indirect labeling method for post-separation detection of chemical compounds
US5071775A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 13, 1986 |
| Grant date | Dec 10, 1991 |
| Priority date | — |
| Expiry date | Aug 13, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/13
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A sensitive and general method of post-separation detection and quantification of chemical compounds is described. The method involves separation of unlabeled compounds by chromatography, electrophoresis or other means and selective binding of the separated compounds to ligands containing highly neutron-activatable elements, followed by neutron irradiation. The neutron-activatable elements are converted to their radiation-emitting isotopes by neutron absorption, and detection is done by autoradiography, fluorography or other means of radiation detection. The theoretical sensitivity of the method is in the attomole (10.sup.-18 mole) range.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.