Method of enrichment of coagulation factors II, VII, IX and X

US5071961A · kind A · utility

Assignee

• Biotest Pharma GmbH · DE

Inventors

• Michael Kraus · Ehrenkirchen, DE
• Wolfgang Moller · Lübeck, DE
• Bertram Eichentopf · Bad Soden, DE

Key dates

• Filing date: Jul 20, 1989
• Grant date: Dec 10, 1991
• Expiry date: Jul 20, 2009

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method of enrichment of coagulation Factors II, VII, IX and X in preparations obtained from plasma, plasma fractions, or other liquids containing the factors, by adsorbing the factor or factors onto a polymeric matrix that carries an .alpha.-hydroxylamine group, and eluting the factors. When the chromatography conditions are appropriate, it is also easy to prepare a highly concentrated Factor IX preparation with a purity of more than 10 U per mg of protein.