Rapid method for determining efficacy of a sterilization cycle and rapid read-out biological indicator
US5073488A · kind A · utility
116Cited by
25References
25Claims
0Family size
Assignee
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Key dates
| Filing date | Nov 29, 1988 |
| Grant date | Dec 17, 1991 |
| Priority date | — |
| Expiry date | Nov 29, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A rapid method of determining the efficacy of a sterilization cycle, and an indicator adapted to perform such method, comprising subjecting to the sterilization cycle a source of active enzyme having activity which correlates with the viability of a microorganism commonly used to monitor sterilization, and incubating the enzyme source, following the completion of the sterilization cycle, with an effective amount of a substrate system capable of reacting with any residual active enzyme to produce a detectable enzyme-modified product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.