Methadone fluorescence polarization immunoassay
US5073629A · kind A · utility
7Cited by
26References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 23, 1989 |
| Grant date | Dec 17, 1991 |
| Priority date | — |
| Expiry date | Jan 23, 2009 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/9486
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence polarization immunoassay for detecting the presence of methadone in a test sample is provided, based upon competition between methadone and a fluorescently labeled tracer for the binding sites on an antibody specific for methadone. The concentration of methadone in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of methadone in the sample. Tracers used as reagents and immunogens used to raise antibodies for use as reagents are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.