Screening for antibodies which bind carbohydrate epitopes of tumor-associated antigens, and uses thereof
US5075218A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 1988 |
| Grant date | Dec 24, 1991 |
| Priority date | — |
| Expiry date | May 24, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/822
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Cells which produce antibodies which bind carbohydrate epitopes of glycoproteins are identified by screening with mucinous body fluids in raw, or preferably partially purified, form. Fine differences in antibody specificity may be detected by further screening with mucinous body fluids treated to alter the carbohydrate epitope interest, e.g., removal of sialic acid from a sialosyl Lewis-a epitope. The selected antibodies may be used in immunopurification, immunodiagnosis and immunotherapy. By this method, antibodies have been found whose binding to carbohydrate epitopes such as sialosyl Lewis-a is relatively insensitive to pH. Such antibodies are of particular value in immunological methods where pH is a consideration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.