Diagnostic kit and method for rapid detection of antibodies
US5077198A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 14, 1988 |
| Grant date | Dec 31, 1991 |
| Priority date | — |
| Expiry date | Apr 14, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Diluent and wash compositions are useful in a rapid and sensitive assay for detecting antibodies, and especially retroviral antibodies, in a biological specimen. The diluent composition is buffered to a pH of 6 to 10 and includes a protein or carbohydrate, a surfactant and a negatively-charged organic compound. The wash composition is buffered to a pH of 5 to 10 and includes a surfactant. These compositions can be included in a diagnostic kit. The method of this invention includes mixing the biological specimen with the diluent composition, forming an immunological complex between ligand and antibodies in the specimen and separating complexed materials from uncomplexed materials using a filtration membrane and a washing step. An enzyme labeled anti-antibody is added to form a ligand-antibody-antibody complex followed by its detection using suitable reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.