Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells
US5077214A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 7, 1989 |
| Grant date | Dec 31, 1991 |
| Priority date | — |
| Expiry date | Jul 7, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/14143
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides an alternative strategy for baculovirus-mediated foreign gene expression: the promoters from immediate-early or delayed-early baculovirus viral genes were used to obtain the continuous expression of foreign genes in stably-transformed insect cells. The IE1 or the 30K promoters from Autographa californica nuclear polyhedrosis virus can drive the continuous expression of a foreign gene in insect cells. IE1-.beta.-galactosidase or 39K-.beta.-galactosidase constructs were used in combination with the IE1-neomycin resistan The Government may have rights in this invention pursuant to a funding agreement with the National Science Foundation (NSF), Grant No. DMB-88 04732.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.