Process for activation of recombinant protein produced by prokaryotes

US5077392A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Rainer Rudolph · Waldshut-Tiengen, DE
• Johannes Buchner · Regensburg, DE
• Helmut Lenz · Tutzing, DE

Key dates

• Filing date: Oct 17, 1989
• Grant date: Dec 31, 1991
• Expiry date: Oct 17, 2009

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/867
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a process for the activation of gene-technologically produced, biologically active proteins expressed in prokaryotes after cell digestion by solubilization under denaturing conditions and reducing conditions and subsequent reactivation under oxidizing and renaturing conditions, wherein working is carried out at a protein concentration of 1 to 1000 .mu.g./ml. and, between the solubilization and the reactivation, a dialysis is carried out against a buffer with a pH value of from 1 to 4 containing 4 to 8 mole/liter guanidine hydrochloride or 6 to 10 mole/liter urea.