Activation of macrophage tumoricidal activity by granulocyte-macrophage colony stimulating factor
US5078996A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1986 |
| Grant date | Jan 7, 1992 |
| Priority date | — |
| Expiry date | Jul 31, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/828
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Macrophages and precursor monocytes are activated to exhibit tumoricidal activity by stimulation solely with granulocyte-macrophage colony stimulating factor. A patient suffering from tumors can be treated by direct administration of therapeutically effective quantities of activated granulocyte-macrophage colony stimulating factor. Homogeneous granulocyte-macrophage colony stimulating factor for use in activating macrophages and monocyte precursors is prepared by recombinant DNA techniques. The gene coding for granylocyte-macrophage colony stimulating factor is isolated and then recombinant protein product expressed in an appropriate expression system. The granulocyte-macrophage colony stimulating factor recovered from the expression system is purified to homogeneity by reverse phase high-performance liquid chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.